Functions as an RNA ligase, in vitro (PubMed:36792600). The ligation reaction entails three nucleotidyl transfer steps (PubMed:36792600). In the first step, the RNA ligase reacts with ATP in the absence of nucleic acid to form a covalent ligase-AMP intermediate and release pyrophosphate (PubMed:36792600). In step 2, the ligase-AMP binds to the nucleic acid and transfers the adenylate to the 5′-PO4 terminus to form an adenylylated intermediate (PubMed:36792600). In step 3, the RNA ligase directs the attack of the 3′-OH on the 5′-phosphoanhydride linkage, resulting in a repaired 3′-5′ phosphodiester and release of AMP (PubMed:36792600). Exhibits selectivity for single-stranded RNA substrates and may not have nick-sealing activity on double-stranded DNA-RNA hybrids (PubMed:36792600). May play a role in maintaining RNA integrity under stress conditions, for example in response to reactive oxygen species (ROS) (PubMed:36792600).

    Matrix Type

    • Plasma
    • Tissue/Cells

    Gene Symbol

    • RLIG1

    UniProt ID

    • Q8N999

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