Our KRAS assay provides quantitative measure of Ras proteins which bind GDP/GTP and possess intrinsic GTPase activity (PubMed:20949621). These proteins play an important role in the regulation of cell proliferation (PubMed:22711838, PubMed:23698361). They also play a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner (PubMed:24623306). Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP). Interaction with SOS1 promotes exchange of bound GDP by GTP.
We perform the KRAS assay using mass spectrometry to achieve high analytical specificity in the measure and quantification of RAS protein levels and characterization of Ras protein interactions in plasma. Our KRAS assays provide rapid insights to support drug discovery projects targeting the KRAS/MAPK pathway.
