Our BRAF kinase assay measures protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Upon stimulation, BRAF phosphorylates MAP2K1 and thereby activates the MAP kinase signal transduction pathway (PubMed:21441910, PubMed:29433126). It also phosphorylates PFKFB2 (PubMed:36402789) and may play a role in the postsynaptic responses of hippocampal neurons (PubMed:1508179). In quiescent cells, BRAF is maintained in an inactive state via an intramolecular interaction between the protein kinase and N-terminal domains. Following mitogen-mediated cell activation, it binds via its RGB domain to active HRAS (GTP-bound) which releases the inhibitory intramolecular interaction between the two domains. This allows the MAP2K1-mediated dimerization of KSR1 or KSR2, and BRAF which activates BRAF.(PubMed:29433126).
We perform the BRAF kinase assay using mass spectrometry to achieve high analytical specificity in the measure and quantification of BRAF kinase protein levels in cells and tissue.
