Learn how Sapient’s extensive quality matrix enables evaluation of sample integrity, sample preparation and handling, automated pipetting, and biomarker extraction, as well as monitoring of chromatographic and mass spectrometry performance at scale and in real time. More than 500 parameters are continuously monitored, with quality metrics that include pooled replicate samples analyzed throughout the study as well as exogenous system performance standards that are introduced, or ‘spiked in’, to each sample.
The Technical Note details a quality analysis of Sapient’s LC-MS workflows applied to assay greater than 40,000 human biosamples in six independent batches as part of a longitudinal human study. Over 35,000 molecular features including metabolites and lipids, were evaluated within the population, making this study among the largest and deepest nontargeted mass spectrometry analyses of humans to date.