Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5′- and 3′-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5′-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5′-RGAAGAAC-3′ (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5′-CGAGGCG-3′ motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. May function as export adapter involved in mRNA nuclear export through the TAP/NXF1 pathway.
